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Fish were rinsed with PBS three times, placed in 1:1 PBS:glycerol, and mounted on bridged coverslips
The Ni-NTA flow-through was concentrated and applied to a Superdex 200 column equilibrated with 25 mM Tris 7.5, 60 mM NaCl and 5 mM DTT
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Green tea polyphenols prevent early vascular aging induced by high-fat diet via promoting autophagy in young adult rats
doi: 10.1016/j.ijpharm.2009.05.029
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