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The combination of the amylin analogue pramlintide and the recombinant human leptin metreleptin caused significant weight reductions ( - 11.5kg) in comparison with monotherapy in a 24-week phase 2 clinical trial [248]

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After being fed HFD for a total of 16-weeks (an appropriate rodent model of DIO and comparable to human obesity [21]) and reaching pre-arrival body weights (~38g), mice were randomized into balanced control and treatment cohorts (n=9/cohort), with similar group average body weight and standard deviation