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Therefore, comprehensive metabolomics-based evaluation of LPS-induced acute kidney and liver injury is necessary

Minihepcidins are rationally designed small peptides that mimic hepcidin activity in mice and may be useful for the treatment of iron overload

Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0

In the presence of hydrogen peroxide, they are converted to agents with increased chelation ability (e.g., hydroxylation of the aromatic ring), which may thus chelate iron more readily

Notably, these developmental outcomes correlate with adverse effects on offspring mitochondrial function, including impaired complex I activity, shifts in the NADH/NAD + ratio, and disrupted cellular metabolism that persist into adulthood and correlate with accelerated biological aging