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Binding of Protein on GST Beads Incubate the soluble cell lysate with 1 ml of GST beads (prewashed two times with 10 ml of GST binding buffer) for 1 hr at 4 C under gentle rotation

S.et al (2014)

doi: 10.3389/fimmu.2023.1239142 104

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Protein lysates were boiled at 95 C for 10 min on a thermomixer (Thermo Fisher Scientific) and sonicated on the Bioruptor (Diagenode) system