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IGF-1 LR3 represents a well-characterised, research-grade analogue of human IGF-1, providing an extended-acting model for the study of growth-factor-mediated signalling

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Y.JinH.ZhangJ.et al (2022)

F.et al (2011a)

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Louis, MO, USA) were incubated with in vitro -translated proteins (5 l) (TnT Quick Coupled Transcription/Translation Systems, L1170, Promega, Madison, WI, USA) or recombinant proteins (50100 ng) (ProteinOne, Rockville, MD, USA) in a buffer containing 20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 2 mM EDTA, 2 mM DTT, 5% glycerol, and 0.4% NP-40 at 4C overnight, followed by extensive washes with a similar buffer containing 150 mM NaCl