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All data presented are representative of at least three independent experiments, as indicated in the figure legends, along with the number of biological replicates

A pH of 7 is perfectly neutral

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The histidine imidazole and the N-terminal amine and lysine side chain give GHK a high intrinsic affinity for copper(II), and as the free apo-peptide it has been studied in preclinical and in vitro research as a copper-binding ligand and as a signaling molecule implicated in extracellular-matrix remodeling, collagen and metalloproteinase regulation, and antioxidant and anti-inflammatory gene-expression pathways