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For dye-based DSF, SYPO Orange (Sigma-Aldrich) was used at a dilution of 1:1000 with a protein concentration 0.1 mg/mL in a final volume of 25 L

[DOI] [PubMed] [Google Scholar] 34.Sharp J.D., Singh A.K., Park S.T., Lyubetskaya A., Peterson M.W., Gomes A.L., Potluri L.P., Raman S., Galagan J.E., Husson R.N