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All of these vectors and each column of these matrices are assumed to be centered around zero and scaled to have a variance of one

10.1038/s41598-018-37652-x 85

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After a three-day culture, the cells were selected in Dulbeccos Modified Eagle Medium (DMEM) supplemented with 10% dialyzed FBS (Thermo Fisher Scientific, Waltham, MA) without pyruvate or uracil