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Quantitative real-time PCR (RT-qPCR) For the RT-qPCR analysis, total RNA was isolated from cells, and cDNA was reverse-transcribed by using M-MLV reverse transcriptase (Promega, Madison, WI, USA)

Histopathological examination Assessment of scaffold biocompatibility The local tissue response post-implantation was evaluated by observing inflammation, necrosis, edema, and congestion during the early and late stages of healing, precisely 2- and 6-weeks post-surgery

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You have a documented clinical reason for a modified formulation

Others stimulate the body's own growth hormone production

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