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These cultures were incubated at 37C with agitation until reaching an OD 600 of 0.8-1.0 for the induction of protein expression with 0.2 mM IPTG at 16C for 16 hours

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Dephosphorylated CopR is transcriptionally inactive and does not induce expression of the pcoBA operon (Hofmann et al

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Recent studies have underscored the role of miRNAs in the down-regulation of inflammation in autoimmune diseases and inflammatory disorders (42)

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