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10.1016/0014-5793(93)80263-T

Following treatment with 0.5 mg/mL LPC, an initial increase in GFAP+ expression is observed over a 72-h period when compared to that of untreated slices, but with further time in culture, no difference in GFAP+ expression is seen between untreated and LPC-treated slices
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doi: 10.36348/sjls.2020.v05i09.007 44 HathcockJNShaoA

Inject the water slowly over 15 to 30 seconds
