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As the test substance diffuses through the agar, it becomes more dilute

Autonomic nervous system dysfunction may explain the multisystem features of fibromyalgia

Reigstad CS, Salmonson CE, Rainey JF 3rd, Szurszewski JH, Linden DR, Sonnenburg JL, Farrugia G, Kashyap PC (2015) Gut microbes promote colonic serotonin production through an effect of short-chain fatty acids on enterochromaffin cells
Prolonging lifespan of the cells could have been due to reactivation of telomerase gene in somatic cells

A neuronal molecular switch through cell-cell contact that regulates quiescent neural stem cells

But accuracy matters more than convenience
