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Among several NO-releasing prodrugs O 2 -(2,4-dinitrophenyl) 1-[(4-ethoxycarbonyl)piperazin-1-yl]diazen-1-ium-1,2-diolate (JS-K) 4 has undergone trials in several animals models and shown promise as a novel prodrug in cancer chemotherapy 5

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Metal-free denatured monomer, which is the point of entry for filament formation in pathological conditions, was produced by the cleavage of the intramolecular disulfide bond and the loss of metal ions

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