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For SRS microscopy, spatially and temporally overlapped pulsed Pump (tunable from 720 to 990 nm, 7 ps, 80 MHz repetition rate) and Stokes (1064 nm, 56 ps, 80 MHz repetition rate, modulated at 8 MHz) beams provided by picoEMERALD (Applied Physics & Electronics) were coupled into an inverted laser-scanning microscope (FV1000 MPE, Olympus) optimized for near-infrared throughput

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The peptide was administered to overweight healthy participants at 1, 3, or 6 mg as a daily subcutaneous injection for 14 days in a phase 1 MAD trial in 2012 (NCT01469923) (62, 63)

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