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Five additional groups were vaccinated three times at three-week intervals with the following formulations: AddaS03 adjuvant at a 1:1 volume ratio in a total of 100 L PBS/mouse, AS01 adjuvant (5 g MPL/5 g QS-21 in a total of 100 L PBS/mouse), metE-DNA (100 g/100 L PBS/mouse), 1 g metE protein combined with AddaS03 adjuvant at a 1:1 volume ratio in a total of 100 L PBS/mouse, and AS01 adjuvant (5 g MPL/5 g QS-21 in a total of 100 L PBS/mouse)

The cells were incubated with the respective probes in growth medium for the indicated time

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Babenko VN, Smagin DA, Kovalenko IL, Galyamina AG, Kudryavtseva NN

It is located within the National Reserve Fauna Andean Eduardo, in the highlands in the department of Potos, near the border with Chile