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To further investigate the KEGG pathways in CM10-CM14, gene set enrichment analysis revealed significant roles in protein folding, cellular signaling, and stress response, highlighting pathways such as Protein processing in endoplasmic reticulum, Glutathione metabolism, and Plant hormone signal transduction. (Fig

The hierarchical heatmap was generated (Fig

Upon comparing control samples in the HEK-293 cell line with these incubated in a 0.1 mM H 2 O 2 solution, statistically significant differences in the levels of several metabolites were identified, including glutamate, acetate, pyroglutamine, glutamine, 3-hydroxybutyrate, lactate, and unknown_1