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The samples were centrifuged (5000 g , 10 min, 4 C) before transferring the supernatant into new tubes

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Eur Rev Med Pharmacol Sci 16(9):11841191 Ohata J, Zvaifler NJ, Nishio M, Boyle DL, Kalled SL, Carson DA, Kipps TJ (2005) Fibroblast-like synoviocytes of mesenchymal origin express functional B cell-activating factor of the TNF family in response to pro-inflammatory cytokines

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For each patient the stimulation index was calculated by dividing the mean counts of the antigen stimulated cultures with the mean counts of the control cell cultures in which no antigen, i.e