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Threshold cycle numbers were transformed using the cycle threshold and relative value method described by the manufacturer and were expressed relative to glyceraldehyde-3-phosphate dehydrogenase, which was used as a housekeeping gene

It was responsible for approximately 9.6 million deaths in 2018, and the high mortality rate of cancer makes it a civilizational disease often termed as a cancer epidemic [11, 12]

It has been attributed to left atrial enlargement and dilation in mitral annulus as the primary mechanism for mitral leaflet malcoaptation (6)