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On day 12, mice were subjected to a 4 h fast period, then deeply anesthetized using isoflurane and trunk blood was collected by decapitation

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Storage guidelines for reconstituted peptides: Refrigerator storage (2-8C): 2-4 weeks recommended maximum Freezer storage (-20C): Possible but not recommended due to freeze-thaw damage Peptides containing Met, Cys, or Trp have shorter shelf life due to oxidation Peptides containing Asn, Gln are more prone to degradation in solution Create multiple aliquots to avoid repeated freeze-thaw cycles if freezing is necessary Note: We recommend using reconstituted peptides within 2 weeks when possible, as potency gradually decreases over time