glutathione s transferase assay principle GST-Pull Down Technology Glutathione transferases as mediators of

Nucleic acids were precipitated from the aqueous fraction overnight at -80C in ethanol containing 150 mM sodium acetate (pH 5.2)

Nonetheless, in a direct comparison of the reactions of lysine, histidine, serine and cysteine with a synthetic keto-epoxide analogue, only Cys adducts were detectable after 1h reaction at 37 C in physiological buffer, and Ohno and co-workers went on to identify the adducts as formed by Michael addition to the conjugated enone

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