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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )

In France, treatment involves the administration of 1,000 g (1 mg) of cyanocobalamin per day for 1 week, followed by 1,000 g (1 mg) per week for 1 month, followed by 1,000 g (1 mg) per month, again, normally for the remainder of the patients lifetime [10]

Zhou L, Bryant CD, Loudon A, Palmer AA, Vitaterna MH, Turek FW

aureus , MIC of chloramphenicol was 8 g/ml

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