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Immunofluorescence staining was performed using primary antibodies against Perilipin-2 (PLIN2, Thermo Fisher Scientific) or HSL (Cell Signaling Technology, Danvers, MA), followed by Alexa Fluor 594- or 488-conjugated anti-rabbit IgG antibodies

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Initial Treatment Dosage: 1000 mcg Daily Protocol Adults with a severe B12 deficiency usually start with 1000 mcg daily for one week

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