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Theriogenology (2012) 78(8):17008.10.1016/j.theriogenology.2012.05.002 32 GuerrieroGCiarciaG

[DOI] [Google Scholar] 88.Santos J.S., Alvarenga Brizola V.R., Granato D

Quantification of GSH using Ellmans assay Cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford protein free lysate

Moreover, bioavailability of metals in free ionic form increases at acidic pH whereas reduced at high pH because of precipitation

It prevents color degradation and enhances the flavour as well

Additionally, we welcome patients from across South Yorkshire, including Rotherham, Wakefield, Sheffield, Leeds and Barnsley