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After blocking with 3% bovine serum albumin BSA for 30 min, GFAP rabbit polyclonal antibody (16825-1-AP, Proteintech) (1:5000) was added and incubated overnight at 4 C

Rozza AL, Moraes TM, Kushima H, Tanomoto A, Marques MOM, Bauab TM, Hiruma-Lima CA, Pellizzon CH: Gastroprotective mechanisms of Citrus lemon (Rutaceae) essential oil and its majority compounds limonene and pinene: involvement of heat-shock protein-70, vasoactive intestinal peptide, glutathione, sulfhydryl compounds, nitric oxide and prostaglandin E2

The default response to a stall is almost never take more it is to check the controllable variables first

5-HT was found to mediate anxiety [146] and showed a moderate reduction of the symptomatology on the 90-item symptoms checklist (SCL-90) and the state scale of the Spielberger StateTrait Anxiety Inventory, suggesting that brain serotonergic pathways are involved in the pathogenesis of anxiety disorders, particularly in agoraphobia and panic disorders [147]