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Supernatant was discarded, and the pellets air-dried and resuspended at a concentration of 12 g/L in sample buffer consisting of 1 Bolt LDS sample buffer (Thermo, Australia), 1 Pierce protease inhibitor cocktail (Thermo, Australia), 1 sample reducing agent (Thermo, Australia) in Milli-Q H 2 O

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A 25-unit draw of reconstituted semaglutide at 1 mg/ml is 0.25 mg

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