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and critical illness with respiratory failure, shock or multi-organ dysfunction was reported in 5% [3]

Poly (lactic acid-co-glycolic acid) (PLGA) copolymers were utilized as carriers to form films (LA@PLGA) via electrospinning, enabling the controlled release of ALA and effectively blocking the production of ROS in damaged hearts

Membranes were incubated with primary antibodies diluted in 5% milk 1X TBST overnight at 4C: anti-Actin (1:3000) (Sigma, A2066), anti-NRF2 (1:1000) (Cell Signaling Technology #12721) and anti-GSR (1:1000) (ThermoFisher, PA5-29945), followed by washing and incubation with HRP-conjugated anti-rabbit IgG (1:2000) (CST#7074) in 5% milk 1X TBST (22C, 1 h)