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In vitro ADP-riboxanation reactions For in vitro reactions, 20 g of purified GST-SEPT9 proteins were incubated with 1 g of GST-OspC3 or its EH/AA mutant and 0.5 g of His-Sumo-CaM in 40 L of a reaction buffer containing 50 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM DTT, 2 mM MgCl 2 , and 1 mM NAD +

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D1-like receptors are stimulatory, where DA binding causes an elevation of cyclic adenosine monophosphate (cAMP) levels, leading to increased activity of protein kinase A (PKA)