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Membranes were blocked with 5% milk in 0.1M TBST and probed with primary antibody: GAPDH (Mouse, Abcam G8795,1:1000) was used as an internal control for the concentration of protein loaded, while anti-MBP (Rat, BioRad MCA4095,1:1000) was used to detect MBP
L-arginine treated animals had IVH size 24.619.53 (p=0.013)
Women should avoid melanotan-2 at high doses due to unpredictable melanocyte stimulation
Retesting every 3-6 months during active protocols helps track changes and guide adjustments
It is commonly paired with other secretagogues for synergistic effects
