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IBMX, forskolin, KN93, H89, BIM-I and rapamycin were prepared as 1000 stocks in DMSO and the final DMSO concentration was adjusted to 0.25% for all conditions tested, including the control

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-arrestins were first recognized for desensitizing GPCRs by uncoupling them from G-proteins and promoting receptor internalization via clathrin-coated pits, but are now known to act as signaling scaffolds themselves, initiating distinct pathways like ERK, JNK, or NF-B activation