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H.SlomaE.et al (2018)

[ 125 I]GLP-1(7-36)NH 2 or [ 125 I]GIP(1-42)OH (2200 Ci/mmol, 95 % purity, final assay concentrations ~0.120.20 nM, PerkinElmer) in assay buffer (2.5 mM MgCl 2 [MilliporeSigma], 1.0 mM CaCl 2 [MilliporeSigma], 0.1% w/v fraction V fatty acid free bovine serum albumin [82-002-4, MilliporeSigma], 0.1% w/v bacitracin [11805, Affymetrix] in 25 mM HEPES [Thermo Fisher Scientific], pH 7.4, final concentrations) was added to peptide in assay buffer (concentration-response curves in DMSO, 3-fold acoustic direct dilution [Echo555, Labcyte], final DMSO concentration in the assay was 0.96%)

* Correspondence: Peijun Liu, [email protected] These authors have contributed equally to this work Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Advances in biased agonism, region-selective targeting, and peripherally restricted analogs now offer potential strategies for selectively modulating central peptide systems without broad CNS disruption

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It requires no refrigeration and has no restrictions on the timing of food or water intake