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First review of the potential renoprotective effects and mechanisms of GLP-1R agonists and DPP-4 inhibitors in preclinical studies and in early clinical studies involving patients with diabetes

J Clin Invest 124: 4473-4488, 2014

Acknowledgments The author declared that there is no conflict of interest

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cAMP accumulation assay Wild-type GLP-1R and GLP-1R mutants were cloned into the expression receptor pcDNA3.1/V5-His-TOPO vector (Invitrogen) at the HindIII and EcoRI sites by using the QuickChange site-directed mutagenesis and a flag-tag was inserted after native signal sequence (see primers in Supplementary Table 2)

While these early effects can be encouraging, the full appetite-suppressing benefits usually develop over the following weeks as the body adjusts to the medication