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In vitro, mouse pancreatic -cells (NIT-1 cells) were exposed to palmitic acid (PA), PA+exendin-4 (Ex-4), PA+GW, PA+GSK0660 (GSK, a PPAR antagonist), or PA+Ex-4+GSK

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We subsequently developed an in vivo technique to assess gut hormone release into the portal vein following colonic infusion of propionate

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They go through relatively small clinical trials, and then theyre released into the real world, where millions of people take them in ways they were never studied

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