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Insert the needle so that its completely covered by skin

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The pipette solution for whole-cell recordings was modified to include an intracellular dye (Alexa Fluor 350 hydrazide dye) and contained: 120 mM K-gluconate, 10 mM KCl, 10 mM HEPES, 5 mM EGTA, 1 mM CaCl 2 , 1 mM MgCl 2 and 2 mM MgATP, and 0.03 mM Alexa Fluor 350 hydrazide dye (pH 7.3)