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Before surgery, blocking GLP-1 did not substantially alter brain connectivity

Recombinant mouse EphA4 Fc chimera protein (R&D Systems, catalog 641-A4-200) was reconstituted in 1 PBS (Genesee, catalog 25-507XB, pH 7.2 0.1), diluted to 3 g/mL using binding buffer, dispensed (100 L) in each well, and rocked on a belly dancer shaker (3 hours at room temperature, RT)

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All analyses were conducted with Review Manager, version 5.4 (Nordic Cochrane Centre, Copenhagen, Denmark)