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Some studies have also found a higher density of GLP-1R-positive cells in the dorsal DMH (dDMH) than the ventral DMH (vDMH), and a higher density of GLP- 1-positive neuronal fibers in the vDMH than the dDMH [72]

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Separate sections were immunostained for PC1/3 using a monoclonal anti-mouse antibody (Abcam, ab3532

The effect of insulin treatment on bladder weight has mostly been studied in a prevention setting, i.e., starting within 1 week after induction diabetes