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Given the pre-existing data on GLP-1 and glucagon, we designed a double-blinded randomised cross-over study, in which we gave volunteers who were overweight short-term intravenous infusions of glucagon (50 ng/kg/min or 14 pmol/kg/min), GLP-1 (0.8 pmol/kg/min), combination of glucagon + GLP-1 at the same doses, or placebo over 45 minutes, and demonstrated that there was a significant increase in resting energy expenditure with glucagon alone which was preserved with the combination of glucagon + GLP-1, whereas GLP-1 did not affect energy expenditure (66)