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Two primary modeling approaches are used for GLP-1 receptor agonists: 2.3

The peak amplitude of a change in fluorescence induced by GLP-1, GLP-1MK-801 or NMDA was calculated by taking an average of ten datapoints from the baseline ( F 0 ) and subtracting this from an average of ten data points from the peak fluorescence ( F : average amplitude peak average amplitude baseline ), which was normalized by dividing by F 0

Calcium deposition assay To determine the calcium content, cells were incubated with 0.5 N HCl for 24 hours at 4C

Clear Instructions for Beginners I had never reconstituted any peptide before and felt anxious about getting the ratios wrong