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To place the nicotine fully in the non-ionic, free-base form, 7 mL of 5 normal NaOH was added, then allowed to wet the sample material for 15 min

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demonstrated that mice chronically exposed to e-cigarette aerosol accumulated elevated levels of DNA adducts in lung, bladder, and heart tissues, accompanied by a marked reduction in DNA repair enzyme activity, highlighting that both genetic integrity and repair capacity are compromised by such exposure (56)

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43 Future work that examines a longer duration of e-cigarette use would be of value to better characterize the impact of these products on COPD outcomes