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The cultures were then grown to the stationary phase in a shaking water bath for 18 h at 30C, after which time the yeast cells were collected, washed with phosphate-buffered saline (PBS), counted by means of a haemocytometer, and adjusted to 10 6 /ml prior to use

Participants were also unable to hold cigarettes and experience tactile sensations which may have influenced their responses

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(19692022) Linked to county attributesTotal U.S., 19692022 counties

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