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For the Promega WGE system this was 30 mM HEPES-KOH (pH 7.6), 200 mM potassium acetate, 3.1 mM magnesium acetate and 7.4 mM DTT, supplemented with 1.2 mM ATP, 0.1 mM GTP, 10 mM creatine phosphate, 80 M of each amino acid and 500 M spermidine

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