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We identified a 13.23-Mb variety-specific SV on chromosome 14, which was present in all G5 group accessions but only in 23% of G4 and 15% of G3 groups, and absent from the G2 group

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The reaction was incubated for 30 min at 40 C, 20 min at 45 C, 20 min at 50 C, 20 min at 55 C, and 20 min at 70 C

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