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Qasim et al

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There are additional animal welfare risks associated with the nose-only method such as the mice suffocating in the tube or complications due to faulty machines or human error

Smoking and Other Respiratory Conditions Linked to Sore Throat Smoking is a leading cause of various respiratory conditions, often resulting in a sore throat as a significant symptom
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The use of electronic cigarettes increases airway resistance, makes inhalation more difficult, and contributes to the production of highly reactive free radicals, causing an increase in oxidative stress, which in turn is linked to mitochondrial dysfunction and reduced nitric oxide bioavailability [77]