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Western Blot Assay After treatment, HBE cells were lysed in RIPA buffer (150 mm NaCl, 50 mm Tris pH 8.0, 1% Triton X-100, 0.5% sodium deoxycholate, and 0.1% SDS, supplemented with protease and phosphatase inhibitors)

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In addition to electronic cigarette initiation and continued use, increased anxiety is also evidenced during nicotine withdrawal (58)

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