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Dysbiosis of lower respiratory tract microbiome are associated with inflammation and microbial function variety

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doi: 10.1371/journal.pone.0302431 90

Amplicons generated ( 600 bp) for each genomic region were resolved in 1.5% agarose gel electrophoresis, cleaned using ChargeSwitch PCR Clean-Up Kit (Thermo Fisher Scientific, Waltham, MA), quantified using Nanodrop 2000 (Thermo Fisher Scientific, Waltham, MA), and Sanger sequenced at the Keck Biotechnology Resource Laboratory, Yale University, New Haven CT, USA, to confirm the amplicon sequences prior to dsRNA synthesis

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Tar: 10 mg Nicotine: 0,8 mg Carbon Monoxide: 10 mg
