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Sample sizes varied based on the specific assay requirements (n = 3 for Western blotting, n = 4 for RT-qPCR, n = 5 for ELISA/Histology), a standard approach in in vivo studies to balance rigor and ethical animal use

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A recent study showed higher levels of the toxic metals Cr, Cd, Pb, Ni, and Al in comparison to e-liquids after several days of use [26]