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Around 1560, Jean Nicot, the French ambassador to Lisbon, popularised the use of tobacco leaves as a cure for carcinoma and ringworm in the court of King Francis II of France

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However, for the sake of full disclosure we note that this study was funded by the Health Research Council of New Zealand (grant 09/195R)

Nicotine captured on Cambridge filter was desorbed using 10 mL of methanol with quinoline as an internal standard, and was analysed using gas chromatography with Thermionic Specific Detector (GC-TSD, Varian Inc.) 21,22,47

Ellard and Gammon, 1976