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In rats induced by advanced glycation end products (AGEs), GA downregulated expressions of MMP-2, MMP-9, and TGF-, thereby inhibiting extracellular matrix (ECM) remodeling and calcification and protecting vascular elasticity

subsequently, we compared differences from the two comparisons against each other in Fig

J Pharmacol Toxicol Methods 88(Pt 1):4045

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The antioxidant function of glutathione is mediated by glutathione peroxidase, which reduces ROS such as hydrogen peroxide and lipid peroxide, resulting in GSSG

Then, the strips were equilibrated in equilibration buffer containing 2% (w/v) SDS, 1% (w/v) DTT, 50 mmol/L Tris-HCl, pH 8.8, 20% (v/v) glycerol, 6 mol/L urea, 0.002% (w/v) bromophenol blue and 1.5% (w/v) iodoacetamide for 15 min at room temperature