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Reconstituted solutions should be refrigerated

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This variation in pO2, in particular, differentially regulates the compartmentalization and functioning of the transcription factors hypoxia-inducible factor-1 (HIF-1) and nuclear factor-B (NF-B)

The reduction usually does not stop at the mixed-disulfide species because the second thiol of DTT has a high propensity to close the ring, forming oxidized DTT and leaving behind a reduced disulfide bond