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Importantly, adequate training and thoughtfulness to details is important to mitigate inadvertent adverse effects on animal health and confounded experimental outcomes when testing the intranasal method of administration [193]
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M.CollinsJ
This is significant since the visualization of a spindle is predictive of embryonic developmental competence (146)
(PubMed) Ziegler D, Hanefeld M, Ruhnau KJ, et al

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