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Spray voltage and capillary temperature were set to 2.8 kV (negative ESI) or 4.0 kV (positive ESI) and to 350 C or 300 C, respectively

A systematic review and meta-analysis demonstrated that pacing interventions lead to a significant reduction in fatigue (Hedges g = -0.52) and an improvement in physical function among ME/CFS patients compared to no treatment or routine care [175]

doi: 10.1128/iai.72.6.3373-3382.2004 253 IsaacDTBerkesCAEnglishBCMurrayDHLeeYNCoadyAet al

These effects are associated with increased expression of the cyclin-dependent kinase inhibitors (CDKNs) p21 and p27, which are negative cell cycle regulators (Pandey et al., 2010)

Freezing creates ice crystals that physically puncture peptide molecules, causing irreversible damage